Makoto Yuasa and Kenichi Oyaizu Pages 1685 - 1697 ( 13 )
Reactive oxygen species (ROS) such as superoxide anion radical (O2-. ) play an essential role on normal cellular growth and homeostasis. However, excess ROS generated by perturbing O2-. homeostasis under various conditions of oxidative stress induce high radical toxicity, resulting in many diseases such as cancer, brain and myocardial infarction, and inflammation. Quantitative analysis of O2-. by a convenient method is a subject of intense research, since most of ROS are derived from O2-. . In situ real-time measurement of O2-. is very important to understand the relevance of ROS to many diseases. Recent progress in electrochemical sensors for the facile detection of O2-. , including biosensors utilizing a variety of metalloproteins as sensing elements for O2-. and very recently developed all-synthetic sensors with a high selectivity for O2-. detection, is reviewed. Emphasis is placed on the possibility of the all-synthetic sensor for convenient in vivo measurement of ROS.
photodynamic therapy, DNA cleavage, BIOSENSORS, Superoxide Dismutase, Iron protoporphyrin, microelectrode
Department of Pure&Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, Noda 278-8510, Japan.