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HR-MAS NMR Spectroscopy: A Practical Guide for Natural Samples

[ Vol. 17 , Issue. 24 ]

Author(s):

Hashim Farooq, Denis Courtier-Murias, Ronald Soong, Wolfgang Bermel, William M. Kingery and AndrĂ© J. Simpson   Pages 3013 - 3031 ( 19 )

Abstract:


High Resolution Magic Angle Spinning (HR-MAS) NMR spectroscopy is a versatile technique that provides high resolution NMR data on samples containing solutions, gels and swellable solids. In addition to providing structural information HR-MAS can also be used to investigate interfaces (for example organic structures at the solid-aqueous soil interface), processes such as swelling/ flocculation, kinetic transfer between gel/liquid phases, as well as conformation and molecular interactions in situ. As such, HRMAS has potential in a diversity of fields including organic, biological, environmental, and medical research. This manuscript focuses on the application of HR-MAS to intact natural samples. A range of 1D and 2D NMR experiments are reviewed and compared in terms of general performance on a range of samples. Therefore, this practical guide and review should be a useful reference and starting point for those wishing to apply HR-MAS NMR. While this article focuses on natural samples, common key 1D and 2D experiments are considered which may be of interest to readers with diverse research interests.

Keywords:

1D NMR, 2D NMR, Algae, High resolution magic angle spinning (HR-MAS), Natural samples, NMR, Soils, Worm tissue.

Affiliation:

Department of Chemistry, University of Toronto, 1265 Military Trail, Toronto, Canada, M1C 1A4.



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